FIG. 4.

Localization of ClfA from S. aureus ISP479C and PS12. Western blot analysis of cell wall extracts and spent culture media precipitates from ISP479C and PS12. An equivalent volume of uninoculated media (BHI) was included as a control. The proteins were probed with antiserum raised against the A region of ClfA. Compared with the parental strain, ClfA was nearly undetectable in the cell wall extracts of PS12. Note the truncated form of the protein detected in the culture supernatants of PS12. TCA, trichloroacetic acid.