Figure 1.

Optimizing expression levels of exogenous miniTurbo fusion proteins in NCI-H295R cells

(A) Immunoblot of PKAc expression after 48 h of doxycycline induction. Top bands represent exogenous miniTurbo-tagged WT PKAc (lane 1), PKAc mutant 1 (lane 2), and PKAc mutant 2 (lane 3). Bottom bands represent native PKAc.

(B) Quantitation of immunoblot in A. Percent expression calculated according to Figure 6. Data are represented as mean ± SEM. No significant differences across conditions by 1-way ANOVA. n ≥ 3. Adapted from Omar et al.³