Skip to main content
. 2023 Jan 4;4(1):101992. doi: 10.1016/j.xpro.2022.101992

Figure 2.

Figure 2

Testing labeling variables

(A) Immunoblot showing effect of doxycycline concentration on PKAc-miniTurbo expression and biotin labeling efficiency.

(B) Immunoblot exhibiting effect of biotin concentration on labeling efficiency.

(C) Immunoblot demonstrating effect of biotin incubation time on labeling efficiency. For PKAc panels (middle) in (A–C), the bottom bands represent native PKAc while the top bands represent exogenous miniTurbo-tagged PKAc. Standard conditions for this protocol are designated by green text in all three experiments.