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Upgulaiton of EMT-associated markers in ICOs upon H/R stress. (a and b) Heatmap is plotted to show the expression of cholangiocyte/epithelial genes and EMT-associated genes in ICOs (n = 4) cultured in normoxia (NR) or 72-h hypoxia (HY) or HY followed by 6-h reoxygenation (RE). (c) Stimulated ICOs were fixed and stained for KRT19, TGF-β, and N-cadherin, along with DAPI staining. Representative fluorescent images are shown.
