Fig. 5.

Increased expression of EMT-associated markers in primary cholangiocytes during clinical liver transplantation IRI. Liver biopsies from non-ischemia living donor graft (n = 1) of ischemic decreased-donor grafts (n = 3) collected during liver transplantation after reperfusion were fixed and stained using multiplex immunofluorescence for the cholangiocyte-specific marker, KRT19, and EMT-associated markers N-cadherin and TGF-β. White arrows indicate the location of small and large bile ducts.