FIG. 1.

(A) Sample Western blots for activated ERK 1 and 2 (upper blot) and total ERK 2 (lower blot) from neutrophils of adults and newborns incubated with or without 5 ng of LPS per ml and the indicated plasma concentrations for 20 min. PMN, polymorphonuclear leukocytes. (B) Neutrophils from five adults and five newborns were incubated with or without plasma (5%) and LPS (5 ng/ml) for 20 min. The mean (± standard error of the mean) level of activated ERK 1 and 2 divided by total ERK 2 determined by densitometric scanning is indicated. (C) Autoradiograph showing phosphorylation of myelin basic protein (MBP) by ERK in the same adult samples shown in panel A.