Table 2.
Primer sequences for RT-PCR.
| Genes | Accession no. | Primer sequence5′-3′ | |
|---|---|---|---|
| Keap1 | MN416132.1 | Forward: | GCCCTCAACAACTGCAT |
| Reverse: | CGGGTCGTAACACTCCA | ||
| Nrf2 | MN416129.1 | Forward: | ATCACGAGCCCTGAAACCAA |
| Reverse: | GGCTGCAAAATGCTGGAAAA | ||
| Maf | XM_040707140.1 | Forward: | AGTCCTGCCGCTTCAAG |
| Reverse: | GTAGGCGTCCCTCTCCC | ||
| CAT | NM_001031215.2 | Forward: | TATCCTTCCTGGTCTTT |
| Reverse: | CATCTGTTCTACCTCCG | ||
| SOD1 | NM_205064.2 | Forward: | ATGTGACTGCAAAGGGAGGA |
| Reverse: | AGCTAAACGAGGTCCAGCAT | ||
| HO-1 | NM_205344.2 | Forward: | GAAAGCTGCCCTGGAGAAAG |
| Reverse: | CCCAGACAGGTCTCCCAAAT | ||
| NQO1 | NM_001277620.2 | Forward: | TCAATGCCGTGCTCTCA |
| Reverse: | CAGCCGCTTCAATCTTC | ||
| GSR | XM_040671422.1 | Forward: | AGTGGCTTGCTGGAGGT |
| Reverse: | GGGTCAGGAGGGCTTTG | ||
| GPX-1 | NM_001277853.3 | Forward: | GACCAACCCGCAGTACATCA |
| Reverse: | GAGGTGCGGGCTTTCCTTTA | ||
| GCLC | XM_040666478.1 | Forward: | AAATGGGACAGGCACAG |
| Reverse: | GGGATCAAACCAGGAAA | ||
| GCLM | NM_001007953.2 | Forward: | TTCGGTCATTATTGCCC |
| Reverse: | ACCTGATTGCTGCTTGG | ||
| GSS | XM_040688004.1 | Forward: | GAACCTCCTACATCCTG |
| Reverse: | CTGACATAGACACCGAA | ||
| Occludin | NM_205128.1 | Forward: | AGCCCTCAATACCAGGATGTG |
| Reverse: | CGCTTGATGTGGAAGAGCTTG | ||
| Claudin1 | NM_001013611.2 | Forward: | AGAAGATGCGGATGGCT |
| Reverse: | AACGGGTGTGAAAGGGT | ||
| Claudin2 | NM_001277622.1 | Forward: | GATACGTGTAGCAGCAGCAG |
| Reverse: | AGCTGGGATTTCTGAGCAGT | ||
| ZO-1 | XM_040680632.1 | Forward: | AAGAGGAAGCTGTGGGTAACTC |
| Reverse: | TGAAGAGTCACCGTGTGTTGT | ||
| β-actin | NM_205518.2 | Forward: | AACCCCAAAGCCAACAG |
| Reverse: | ACAGGGACAGCACAGCC | ||