Table 5.
Effect of glycine on the serum antioxidant status of heat-stressed broilers.
| Effect of glycine under HT |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Treatments1 |
CON vs. HT |
P value |
||||||||
| Items | CON | HT | 0.5%Gly | 1.0%Gly | 2.0%Gly | SEM | P value | Main effect | Linear | Quadratic |
| H2O2 (mmol/L) | 30.94 | 43.23 | 26.97 | 28.25 | 25.40 | 2.16 | 0.023 | 0.001 | 0.001 | 0.010 |
| MDA (nmol/mL) | 3.27 | 4.26 | 3.30 | 3.29 | 2.78 | 0.21 | 0.044 | 0.043 | 0.008 | 0.567 |
| CAT (U/mL) | 3.33 | 5.10 | 5.77 | 5.34 | 4.37 | 0.22 | 0.005 | 0.342 | 0.596 | 0.092 |
| SOD (U/mL) | 130.75 | 115.33 | 200.76 | 192.55 | 235.43 | 16.98 | 0.651 | 0.046 | 0.01 | 0.498 |
| GPX (U/mL) | 834.60 | 821.89 | 902.17 | 913.44 | 1015.01 | 22.85 | 0.766 | 0.017 | 0.002 | 0.783 |
| T-AOC (U/mL) | 11.97 | 7.44c | 13.14 | 17.93 | 10.59 | 1.09 | 0.016 | 0.001 | 0.052 | <0.001 |
| GSH (μmol/L) | 29.74 | 15.69 | 18.10 | 21.16 | 18.22 | 1.19 | 0.003 | 0.479 | 0.311 | 0.310 |
| GSSG (μmol/L) | 15.35 | 15.37 | 27.15 | 21.05b | 15.52 | 1.15 | 0.991 | <0.001 | 0.302 | <0.001 |
Abbreviations: CAT, catalase; GPX, glutathione peroxidase; GSH, glutathione; GSSG, oxidized GSH; H2O2, hydrogen peroxide; MDA, malondialdehyde; SOD, superoxide dismutase; T-AOC, total antioxidant capacity.
1
CON: basal diet and raised under thermoneutral conditions; HT: basal diet and raised under cyclic high-temperature conditions; 0.5% Gly, 1.0% Gly, 2.0% Gly: basal diet supplemented with 0.5%, 1.0%, 2.0% glycine under cyclic high-temperature conditions (n = 6).