Figure 1.

WEE1 inhibition leads to rapid large-scale alterations in the phosphoproteome

(A) Experimental and computational workflow overview. Two datasets are generated, a time-resolved (20, 40, 60, 90 min n = 3) and single time point (90 min n = 4).WEE1i indicates adavosertib treatment, and n the number of replicates in each dataset.

(B) Bar plot of detection summary of phosphoproteomic datasets. Number phosphoproteins, phosphopeptides, and phosphorylation sites for individual and combined datasets are shown.

(C) Plot of CDK inhibitory t-loop T14 and Y15 phosphorylation quantification Z-scores are adjusted so that the first time point is equal to zero. Black bars represent the error on the adjusted Z score.

(D) Illustration of T-loop inhibitory phosphorylation of CDK1 and CDK2 by WEE1.

(E) Significantly altered phosphopeptides at different time points were determined by statistical testing. False discovery rate (FDR) < 0.05.