Figure 6.

Adavosertib triggers phosphorylation of multiple clustered sites in DNA replication and repair factors

Dendrogram visualizing detected significantly (FDR <0.01) altered phosphopeptides for proteins involved in DNA repair (A) and replication. (B) Dot size indicates the logFC after 90 min of WEE1 inhibition. Plots showing the phosphorylation pattern of (C) MDC1, (D) 53BP1, (E) RIF1, (F) MCM4, and (G) ORC1. The logFC (90 min) of a phosphosite is indicated on the y axis and the amino acid sequence on the x axis. Significantly altered phosphorylation sites are marked with a label indicating the phosphorylated residue position on the protein. Sites are merged from datasets 1 and 2.