Antibodies |
|
γH2AX |
Merck |
Cat# PC130; RRID:AB_2238184
|
53BP1 |
Novus Biologicals |
Cat# NB100-304, RRID:AB_10003037
|
BRCA1 |
Santa Cruz |
Cat# sc-6954, RRID:AB_626761
|
RPA |
Millipore |
Cat# MABE285, RRID:AB_11205561
|
CDK substrate |
Cell Signaling Technology |
Cat# 9477, RRID:AB_2714143
|
CDK pY15 |
Cell Signaling Technology |
Cat# 9111, RRID:AB_331460
|
CDK1 |
Cell Signaling Technology |
Cat# 77055, RRID:AB_2716331
|
Actin |
Millipore |
Cat# MAB1501, RRID:AB_2223041
|
HRP anti-mouse |
Vector Laboratories |
Cat# PI-2000, RRID:AB_2336177
|
HRP anti-rabbit |
Vector Laboratories |
Cat# PI-1000, RRID:AB_2336198
|
|
Chemicals, peptides, and recombinant proteins |
|
RO-3306 |
CalBioChem |
Cat# 217699; CAS:872,573-93-8 |
CDK2 inhibitor II |
Santa Cruz |
Cat# sc-221409; CAS:222,035-13-4 |
Adavosertib/MK-1775/AZD1775 |
SelleckChem |
Cat# S1525; CAS:955,365-80-7 |
Hydroxyurea |
Merck |
Cat# H8627; CAS:127-07-1 |
TMTpro™ 16plex Label Reagent Set |
Thermo Fisher |
Cat# A44520
|
|
Critical commercial assays |
|
MagReSyn® Ti-IMAC HP |
LabLifeNordic |
Cat# MR-THP002 |
|
Deposited data |
|
Proteomics data |
This paper |
MassIVE: PXD036374; MassIVE: PXD036373
|
Custom code used for proteomics data |
This paper |
Zenodo: https://doi.org/10.5281/zenodo.6344325
|
QIBC data and analysis code |
This paper |
Zenodo: https://doi.org/10.5281/zenodo.7022473
|
|
Experimental models: Cell lines |
|
U2OS |
ATCC |
HTB-96 |
MFC10A |
ATCC |
CRL-10317 |
RPE |
ATCC |
Laboratory of Krister Wennerberg |
OVCAR3 |
ATCC |
Laboratory of Krister Wennerberg |
OVCAR8 |
ATCC |
Laboratory of Krister Wennerberg |
|
Software and algorithms |
|
Visual Studio Code |
Microsoft |
https://visualstudio.microsoft.com |
R project |
N/A |
https://www.r-project.org |
Spotfire 11 |
Tibco |
RRID:SCR_008858 |
ProteomeDiscoverer |
Thermo Fisher |
RRID:SCR_014477 |
Tidyverse |
(Wickham et al., 2019)75
|
https://tidyverse.tidyverse.org/ |
ggprism |
(Charlotte Dawson 2021) |
https://doi.org/10.5281/zenodo.4556067 |
ggplot2 |
(Wickham et al., 2016) |
https://ggplot2.tidyverse.org |
ClusterProfiler |
(Wu et al., 2021)74
|
https://doi.org/10.1089/omi.2011.0118 |
VennDiagram |
(Chen and Boutros, 2011)76
|
https://cran.r-project.org/web/packages/VennDiagram/index.html |
OmnipathR |
(Türei et al., 2016)25
|
https://omnipathdb.org/ |
Mfuzz |
(Kumar and Futschik, 2007)70
|
http://mfuzz.sysbiolab.eu/ |
ggseqlogo |
(Wagih, 2017)71
|
https://omarwagih.github.io/ggseqlogo/ |