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. 2022 Oct 10;13(1):70–84. doi: 10.1158/2159-8290.CD-22-0489

Figure 2.

Figure 2. HPV42 induces transformation in vitro and tumor growth in vivo. A, Growth curves of HPKs transduced with the full genome of HPV11 (low-risk), HPV16 (high-risk), and HPV42. B, CV staining of MEF cells at day 14 after transduction. Scale bar, 100 μm. C, HPV42-E7 protein interaction partners identified in BioID proximity-labeling experiment. Pathway members of GO-term G1–S transition (red) and HIPPO pathway (blue) are labeled. D, E2F reporter assay in U2OS cells transduced with E7 proteins of HPV11, 16, or 42. ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA, Tukey multiple comparisons test). E, (Top) GSEA of MSigDB hallmark gene set and (bottom) MSigDB curated gene set comparing HPV42-E7 vs. empty vector (EV) transduced HPK cells. NES, normalized enrichment score. F, IHC for YAP1 in a DPA tumor. Scale bar, main: 50 μm; inset: 3 mm. G, Individual tumor growth of EV, or E7 of HPV11-, HPV16-, or HPV42-transduced HaCaT cells in NSG mice. Each group consists of 5 mice, i.e., 10 tumors.

HPV42 induces transformation in vitro and tumor growth in vivo. A, Growth curves of HPKs transduced with the full genome of HPV11 (low-risk), HPV16 (high-risk), and HPV42. B, Crystal violet staining of MEF cells at day 14 after transduction. Scale bars, 100 μm. C, HPV42-E7 protein interaction partners identified in BioID proximity-labeling experiment. Pathway members of gene ontology terms G1–S transition (red) and HIPPO pathway (blue) are labeled. FC, fold change. D, E2F reporter assay in U2OS cells transduced with E7 proteins of HPV11, 16, or 42. ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA, Tukey multiple comparisons test). E, Gene set enrichment analysis (GSEA) of the Molecular Signatures Database (MSigDB) hallmark gene set (top) and MSigDB curated gene set (bottom) comparing HPV42-E7 versus EV-transduced HPK cells. dev., development; NES, normalized enrichment score. F, IHC for YAP1 in a DPA tumor. Scale bar, main: 50 μm; inset: 3 mm. G, Individual tumor growth of EV- or E7 of HPV11-, HPV16-, or HPV42-transduced HaCaT cells in NSG mice. Each group consists of 5 mice (i.e., 10 tumors).