Figure 1.

Palb activation of ACLY is dependent on AKT. (A) MDA-MB-231 and Panc1 cells were treated with 0.1% DMSO or 1 µM P for 96 h. Cellular protein was quantified using Bradford assays and equal amounts of protein were separated by SDS-PAGE. (B) MDA-MB-231 or Panc1 cells were treated with 0.1% DMSO and NT RNA as a control. Cells treated with 1 µM Palb for 96 h were also subjected to AKT knockdown. Protein analysis, western blotting and antibodies utilized was as described in the Materials and methods. Results shown were repeated twice, and Palb, palbociclib; NT, non-targeting; ACLY, ATP citrate lyase; Rb, retinoblastoma; RNAi, RNA interference; p, phosphorylated.