Figure 4.

The combination of Palb and BA reduces viability of breast cancer cells grown in 3D cell culture. MDA-MB-231 cells were grown in 3D cell culture and treated with 0.1% DMSO, Palb (1 µM), BA (25 µM) or the combination P + BA for 96 h. (A) Cells in Matrigel formed spheres in 7 days and then were subjected to treatments. Cell viability was measured using CellTiter-Glo 3D Cell Viability Assay. (B) Cells were plated in ultra-low attachment plates that facilitate 3D sphere formation. Cell toxicity after treatment was measured using the CellTox Green Cytotoxicity Assay using Fluorescence (Ex:485 nm Em:520 nm). Experiments were repeated twice with n=8. Error bars represent standard deviation of the mean. One-way ANOVA with Tukey's post hoc test was used to determine statistical significance **P<0.01 compared with Palb or BA treatment alone. Palb, palbociclib; BA, bempedoic acid.