Figure 6.

BA inhibits invasion in various cancer cell types. (Aa) HT1080 cells were treated with 0.1% DMSO or 25 µM BA, followed by assays as described in the Materials and methods. Statistical analysis was performed using unpaired Student's t-test. **P<0.01 compared with DMSO control in the presence of FBS. (Ab) Detection of the mesenchymal markers Snail and Slug by western blotting. Results shown were repeated twice. (Ba) Panc1 cells were treated with 0.1% DMSO or 25 µM BA, followed by assays as described in the Materials and methods. Statistics were performed as in Aa. (Bb) Detection of the mesenchymal markers Snail and ZEB1 through western blotting. (Ca) MDA-MB-231 cells were treated with 0.1% D or 1 µM P for 96 h followed by invasion assays. One-way ANOVA with Tukey's post hoc test was used for statistical analysis. **P<0.01 compared with all other treatments. Western blotting analysis was performed in (Cb) using antibodies against markers of the epithelial phenotype (E-cadherin) or the mesenchymal phenotype (N-cadherin, Vimentin, ZEB1 and Slug). β-actin was used as a loading control. Results shown were repeated twice. ZEB1, zinc finger E-box-binding homeobox 1; BA, bempedoic acid; Palb, palbociclib.