Silencing of HOTTIP alleviates inflammation in asthmatic mice. (A) The expression of HOTTIP was measured in mice treated with OVA or/and HOTTIP knockdown by qRT-PCR. (B-E) The counts of total cells (B), eosinophils (C), macrophages (D), and lymphocytes (E) were detected in bronchoalveolar lavage fluid (BALF) from mice treated with OVA or/and HOTTIP knockdown. (F-I) Levels of IgE (F), Interleukin (IL)-4 (G), IL-5 (H), and IL-13 (I) were determined in BALF from mice treated with OVA or/and HOTTIP knockdown. *P < 0.05, **P < 0.01, ***P < 0.001 of one-way analysis of variance (ANOVA) with Tukey test.