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. Author manuscript; available in PMC: 2023 Jan 9.
Published in final edited form as: ACS Comb Sci. 2018 Oct 30;20(11):653–659. doi: 10.1021/acscombsci.8b00101

Figure 1. Theoretical Basis for Multiplexing using Spectral Imaging and Combinatorics (MuSIc).

Figure 1.

A. Example arrangement of data for a three probe (m=3) setup in terms of the linear unmixing equation. Emission spectra data (μ) of a mixture are arranged vertically, stacked by emission wavelength and excitation channel (indicated by color and background highlighting). Each column of the reference matrix is the emission spectra of a probe in isolation, arranged in the same way. B. Example data for three probe setup involving a teal fluorescent protein (mTFPI), a yellow fluorescent protein (mVenus), and their covalent fusion (mTFPI-m Venus). Two excitation channels are used, 430 and 505 nm, and fluorescence emission spectra measured.