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. Author manuscript; available in PMC: 2023 Jan 9.
Published in final edited form as: Cell Rep. 2022 Nov 22;41(8):111675. doi: 10.1016/j.celrep.2022.111675

Figure 3. Degradation of LBR1600* depends on the E3 ligases RNF5 and HRD1, TMEM33, and the E2 ubiquitin-conjugating enzymes, Ube2G2 and Ube2D3.

Figure 3.

(A) LBR1600*S11 turnover was assessed in respective KO cells using a CHX-chase assay. Lysates from indicated time points were harvested and analyzed using immunoblotting.

(B) Quantification of CHX assay in (A) via densitometry. The plot shows the mean of three independent experiments ± SD.

(C) RNF5 KO cells were transfected with siRNAs for depleting HRD1 for 48 h, and CHX assays were performed as described in (A).

(D) Quantification of CHX assay in (C). The plot shows the mean of three independent experiments ±SD.