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. 2022 Nov 7;24(1):e54042. doi: 10.15252/embr.202154042

Figure 1. ZBTB2 links p53 deficiency to HIF‐1 activation.

Figure 1

  1. The indicated cells co‐transfected with p5HRE‐Luc and either pEF6/ZBTB2 (ZBTB2) or pEF6/myc‐His B (empty vector: EV) were cultured under the indicated oxygen conditions for 24 h for the luciferase assay.
  2. U2OS cells co‐transfected with p5HRE‐Luc and the indicated siRNA were cultured under the indicated oxygen conditions for 24 h for the luciferase assay.
  3. HCT116 p53−/− cells co‐transfected with p5HRE‐Luc, either pEF6/ZBTB2 (ZBTB2) or pEF6/myc‐His B (EV), and either pcDNA3/p53 (at two concentrations: + and ++) or pcDNA3 (EV), were cultured under the indicated oxygen conditions for 24 h for the luciferase assay and western blotting using the indicated antibodies.
  4. HCT116 p53+/+ cells co‐transfected with p5HRE‐Luc, either pEF6/ZBTB2 (ZBTB2) or pEF6/myc‐His B (EV), and pcDNA3/p53 R175H, R248W, R273H, or pcDNA3 (EV), were cultured under the indicated oxygen conditions for 24 h for the luciferase assay.

Data information: Mean ± s.d. The number of technical replicates in all of the experimental groups was 3, and the reproducibility of the results was confirmed at least three times by biologically independent experiments (A–D). N.S.: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student's t‐test (A–D).

Source data are available online for this figure.