Figure EV1. Relationship between p53 and HIF‐1.

• A–C
  U2OS cells transfected with p53‐Luc (B; #219083, Agilent Technologies) or p5HRE‐Luc (C) were treated with DMSO (for control) or 2.5 μM of Nutlin‐3a, cultured under < 0.1% oxygen conditions for 24 h, and subjected to western blotting using the indicated antibodies (A) and luciferase assays (B, C). pRL‐CMV was used as an internal control (B, C).

Data information: Mean ± s.d. The number of technical replicates in all of the experimental groups was 3 (B, C), and reproducibility of the results was confirmed at least three times by biologically independent experiments (A–C) = 3, *P < 0.05, **P < 0.01, Student's t‐test.

Source data are available online for this figure.