Figure EV3. The ZBTB2–HIF‐1 axis promotes invasion and metastasis of p53‐deficient cancers.

• A
  HeLa cells, whose p53 activity is usually suppressed, were transiently transfected with either pEF6/ZBTB2 (ZBTB2) or pEF6/myc‐His B (empty vector: EV) and with either pcDNA3/p53 (p53) or pcDNA (EV), cultured under < 0.1% oxygen conditions for 24 h, and subjected to qRT–PCR to quantify the mRNA levels of the indicated genes. Mean ± s.d. The number of technical replicates in all of the experimental groups was 3. N.S., not significant, *P < 0.05, ***P < 0.001, ****P < 0.0001, Student's t‐test.
• B–D
  Representative image of the invasion assay using the matrigel‐coated transwell chamber in Fig 2D (B), Fig 2E (C), and Fig 2F (D). Arrowheads indicate invading cells. Scale bar, 100 μm.
• E
  Representative images of lungs with metastatic tumors in Fig 2G. Red ROIs represent metastatic colonies in each lung.

Data information: Reproducibility of the result was confirmed at least three times by biologically independent experiments (A–E).

Source data are available online for this figure.