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. 2022 Nov 7;24(1):e54729. doi: 10.15252/embr.202254729

Figure EV3. Phenotypic analysis of CMO mice and CMO mice lacking MyD88.

Figure EV3

  • A
    Y‐axis indicates IL‐1β (pg/ml) in BM and paws isolated from WT (blue symbols) and symptomatic CMO (orange symbols) mice. Each symbol represents one biological replicate (mouse). At least five mice were used per group in two separate experiments. Data represent mean ± s.d. Statistical significance was assessed using two‐tailed Student's t‐tests (*P < 0.05, ns, not significant).
  • B
    IkB‐α levels in HSCs isolated from WT or CMO mice. Y‐axis indicates IkB‐α mean fluorescence intensity (MFI). Values are normalized to the average of WT levels. Each symbol represents one biological replicate (mouse). At least six mice were used per group in two separate experiments. Data represent mean ± s.d. Statistical significance was assessed using two‐tailed Student's t‐tests (ns, not significant).
  • C
    Percentage of distinct BM subpopulations in distinct murine lines. In this figure, each symbol represents one mouse (biological replicates). At least five mice were used per group in three separate experiments. Data represent mean ± s.d. Statistical significance was assessed using two‐tailed Student's t‐tests (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant).
  • D
    IL‐1β levels in BM and paws. Y‐axis indicated IL‐1β (pg/ml) in BM and paws isolated from WT (blue symbols) and symptomatic CMO (orange symbols) mice. Each symbol represents one biological replicate (mouse). At least four mice were used per group in two separate experiments. Data represent mean ± s.d. Statistical significance was assessed using two‐tailed Student's t‐tests (*P < 0.05, **P < 0.01, ns, not significant).
  • E
    Percentage (left panel) and absolute number (right panel) of LKS in BM. Each symbol represents one biological replicate (mouse). At least eight mice were used per group in three separate experiments. Data represent mean ± s.d. Statistical significance was assessed using two‐tailed Student's t‐tests (*P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant).
  • F
    Tri‐lineage reconstitution upon transplantation of CMO and CMO MyD88−/− HSCs. The x‐axis indicates the dose of donor cells. The y‐axis indicates the percentage of cells defined as Gr1+ CD11b+ myeloid cells (green), B220+ B‐cells (red), and CD3+ T‐cells (blue). Each column represents values for one mouse.