Figure 1. IFN‐I‐induced XAF1 expression is negatively correlated with the Irf7 mRNA level.

• A, B
  mRNA levels of IFN‐I (A) and IRF7 (B) in virus‐stimulated WT BMDMs were measured by qRT–PCR at the indicated time points.
• C
  WT BMDMs were stimulated with SeV or VSV‐WT (MOI = 1) for 9 h and treated with MG132 2 h before harvest. The indicated proteins in whole‐cell lysates were detected by IB.
• D
  WT BMDMs were stimulated with SeV for the indicated time course, and whole‐cell lysates were subjected to IP using an anti‐IRF7 antibody. Cell lysates were also subjected to direct IB, and actin was used as a loading control.
• E
  Schematic of the experimental procedure for RNA‐seq and MS.
• F
  Left panel, KEGG analysis of IRF7‐interacting proteins as determined by MS; middle panel, Venn diagrams illustrating the overlap between DEGs identified through analysis of the RNA‐seq and MS results; right panel, heatmap showing overlapping DEGs.
• G
  Abundant XAF1 mRNA expression was induced by different influenza viruses at an MOI of 1 for 6 h in PBMCs obtained from healthy donors (n = 64) as measured by qRT–PCR.
• H, I
  The mRNA (H) and protein (I) levels of XAF1 in virus‐induced WT BMDMs were measured throughout the indicated time course by qRT–PCR and IB, respectively.
• J, K
  qRT–PCR analysis of XAF1 mRNA in BMDMs derived from IFNAR‐KO mice (J) and STAT1‐KO mice (K).

Data information: All data are representative of at least three biologically independent experiments. Data from the qPCR assay are presented as the fold change relative to the Actin mRNA level. The data are presented as the means ± SDs. The significance of differences was determined by t‐tests. *P < 0.05, **P < 0.01, and ***P < 0.005.

Please see Appendix Fig S1 for information regarding replicates, quantification, and statistical evaluation for biochemical data in this figure.

Source data are available online for this figure.