Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 9;12:1. doi: 10.1186/s40035-022-00334-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Overexpression of p85S6K ameliorates cognitive decline and spine deficits in AD mice. a–f Morris water maze was carried out to assess spatial learning and memory. a Latency of WT and APP/PS1 mice to locate the hidden platform in the training period. F_{(1, 39)} = 97.50, P < 0.0001 for genotype effect, F_{(1, 39)} = 14.48, P = 0.0005 for p85S6K overexpression effect. b The average speed of WT and APP/PS1 mice in the training period. c The time spent in the target quadrant in the probe test. F_{(1, 39)} = 6.731, P = 0.0133 for genotype effect, F_{(1, 39)} = 9.915, P = 0.0013 for p85S6K overexpression effect. d The average speed of WT and APP/PS1 mice in the probe test. e The number of platform crossings of WT and APP/PS1 mice in the probe test. F_{(1, 39)} = 4.522, P = 0.0398 for genotype effect, F_{(1, 39)} = 7.984, P = 0.0074 for p85S6K overexpression effect. f Representative swimming trajectories in the probe test. The green circle represented the location of the hidden platform. n = 11 mice for WT-Ctl, WT-p85S6K OE, and APP/PS1-Ctl groups; n = 10 for the APP/PS1-p85S6K OE group. g, h The expression levels of p-Ser845 GluA1 and total GluA1 in the hippocampus after injection of AAVs expressing p85S6K or control AAVs. n = 5 mice per group. Left panel in h: F_{(1, 16)} = 4.731, P = 0.0450 for genotype effect, F_{(1, 16)} = 5.540, P = 0.0317 for p85S6K overexpression effect; right panel in h: F_{(1, 16)} = 6.906, P = 0.0183 for genotype effect, F_{(1, 16)} = 0.1139, P = 0.7401 for p85S6K overexpression effect. i, j Spine density in the hippocampus of WT and APP/PS1 mice after overexpression of p85S6K. Scale bars, 200 μm (left panel) and 1 μm (right panel). n = 9 slices from 4 mice per group. F_{(1, 32)} = 14.97, P = 0.0005 for genotype effect, F_{(1, 32)} = 16.22, P = 0.0003 for p85S6K overexpression effect. Data are presented as mean ± SEM. Three-way ANOVA (a, b) or two-way ANOVA (c, d, e, h, j) followed by Tukey's or Bonferroni's test. *P < 0.05, **P < 0.01, ****P < 0.0001. Ctl control, i.e., mice injected with control AAVs. OE overexpression