Figure 6.

JZL184 results in long-term alleviation of conditioned fear responses. (A) Timeline of experiment. Mice received Veh or JZL184 injections 2 hours before 2MT exposure. Mice were tested for contextual fear expression 2 to 6 days later (Veh: n = 18; JZL184: n = 17). Unpaired, two-tailed t test performed for all analyses. (B) JZL184 has no effect on total distance traveled. (C) JZL184 during 2MT exposure decreases conditioned freezing. (D) JZL184 decreases the total number of conditioned freezing episodes. (E) JZL184 has no effect on time spent in the far zone. (F) JZL184 has no effect on % distance spent in the far zone. (G) Schematic of experimental timeline. All mice were exposed to 2MT. Mice were administered Veh or JZL184. One day later, they were tested for contextual fear expression (Veh: n = 9; JZL184: n = 10). (H) JZL184 before context testing has no effect on total distance traveled. (I) JZL184 has no effect on conditioned freezing during context test. (J) JZL184 does not alter the total number of freezing episodes. (K) Schematic of zones and representative heat map of Veh-treated (left) and JZL184-treated (right) animal, demonstrating that JZL184 administration before context testing decreases conditioned avoidance. (L) JZL184 decreases time spent in the far zone. (M) JZL184 decreases % distance traveled in the far zone. *p < .05; **p < .01. 2MT, 2-methyl-2-thiazoline; Veh, vehicle.