Figure 4.

A reproducible, penetrating (transecting) lesion can be introduced into the cerebellar tissue slice at 8 days post-slice derivation.

Representative images of cerebellar tissue slices (250 µm thickness) taken immediately post lesioning at 8 days post slice derivation (A) and at 3 days post-lesion (B: dotted line indicates lesion margin). Representative fluorescent micrographs of the lesion site at 7 days following transecting injury (C, D) display pathological responses consistent with neurological injury. Areas of upregulated GFAP-immunoreactive cells are evident at the lesion margin (C, arrows). Short-range growth of Tuj-1-immunoreactive axons into lesion areas is evident (D). FITC: green; Cy3: red. Scale bars: 100 µm. GFAP: Glial fibrillary acidic protein; Tuj-1: beta tubulin III.