Figure 6 .

Quercetin induces autophagy and apoptosis in HL-60 cells through the AMPK/mTOR signal pathway

HL-60 cells were incubated with quercetin with or without compound C for 48 h. Proteins were isolated and subjected to western blot analysis using indicated antibodies and quantified using Image-pro plus 6. (A) One of four independent experiments is shown. p-AMPK/AMPK, p-mTOR/mTOR and LC3 II/I ratios were expressed in arbitrary units. n=4 for each group. (B) Caspase-3, Bcl-2 and BAX proteins were detected by western blot analysis and expressed in arbitrary units. n=3 for each group. Data are presented as the mean±SD. * P<0.05, ** P<0.01.