FIG. 2.

Down-regulation of GPI-PLD mRNA by oxidative stresses. (A) Time course response of GPI-PLD mRNA to H₂O₂ stimulation. RAW 264.7 cells were untreated (controls) or treated with 0.5 mM H₂O₂. Samples were taken at 1, 4, and 7 h, and total RNA was prepared and analyzed by Northern blotting. The results are representative of two independent Northern analyses. (B) MQ induces down-regulation of GPI-PLD mRNA. RAW 264.7 cells were stimulated with 50 μM MQ for 1 and 4 h. Cells without MQ were tested in parallel. Total RNA was prepared and analyzed by Northern blotting using 0.8% RNA denaturing gels. The results are representative of two independent Northern analyses.