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. 2022 Oct 2;112(2):583–596. doi: 10.1111/tpj.15957

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© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Validation of Smar2C2 TSS using epigenomic data.

(a) The expected orientation of epigenomic data relative to the transcription start site (TSS) in plants with accessible chromatin identified via ATAC‐seq (gray) present upstream in the promoter, histone modifications of transcription initiation H3K56ac (pink) and H3K4me3 (purple) directly downstream, and histone modifications of transcription elongation H3K36me3 (red) and H3K4me1 (burgundy) further downstream in the gene body. (b) Heat maps of epigenomic and CAGE data centered on the primary TSS identified with Smar2C2 show that these histone modification patterns are consistent across the entire genome, with genes ranked by the volume of ChIP data.