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. 2022 Feb 21;54(3):332–339. doi: 10.3724/abbs.2022018

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© The Author(s) 2021.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Overexpression of H19 inhibited H ₂O ₂-induced cochlear hair cell apoptosis

(A) CCK8 assay for cell viability of HEI-OC1 cells infected with LV-H19 and LV-NC respectively. (B) ATP content in HEI-OC1 cells infected with LV-H19 and LV-NC respectively. (C,D) Representative JC-1 fluorescence micrographs in HEI-OC1 cells infected with LV-H19 and LV-NC respectively. (E,F) Representative ROS fluorescence micrographs in HEI-OC1 cells infected with LV-H19 and LV-NC respectively. (G,H). Annexin V/PI staining for cell apoptosis in HEI-OC1 cells infected with LV-H19 and LV-NC respectively. Data were shown as the mean±SD of three independent experiments. *P<0.05 vs control, and #P<0.05 vs LV-NC.