Figure4 .

H19 inhibited H ₂O _2--induced cochlear hair cell apoptosis via regulating miR-653-5p/SIRT1 axis

HEI-OC1 cells were co-transfected with LV-H19 and miR-653-5p mimic or SIRT1 interference sequence, and then stimulated with H2O2. (A) CCK8 assay for HEI-OC1 cell viability. (B) ATP content in HEI-OC1 cells co-transfected with LV-H19, miR-653-5p mimic and si-SIRT1. (C,D) Representative JC-1 fluorescence micrographs of HEI-OC1 cells co-transfected with LV-H19, miR-653-5p mimic and si-SIRT1. (E,F) Representative ROS fluorescence micrographs of HEI-OC1 cells co-transfected with LV-H19, miR-653-5p mimic and si-SIRT1. (G,H) Annexin V/PI staining for cell apoptosis in HEI-OC1 cells co-transfected with LV-H19, miR-653-5p mimic and si-SIRT1. Data were shown as the mean±SD of three independent experiments. #P<0.05 vs LV-NC, &P<0.05 vs mimic NC, and $P<0.05 vs siRNA.