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Chemoenzymatic synthesis of HS mimetics bearing three NS domains separated by well‐defined NA domains. A) Preparation of alkyne modified compounds 28, 29, and 30 with two NS domains separated by a NA domain of defined length. B) Structure of acceptors 3, 4, and 5 with terminal azide group. C) Assembly of 31–33 having three NS domains separated by two NA domains via CuAAC click reaction.
