FIGURE 1.

Study design. Cells were incubated and differentiated in osteogenic culture medium (OCM) in 2D flasks: Adipose‐derived stem cells (ADSC) differentiated for 7 days (7D‐ADSC) or 14 days (14D‐ADSC). ADSC in expansion medium (MesenPRO) were incubated as control, undifferentiated cells (U‐ADSC). Results from flow cytometry and ARS were referred to these 2D cultures. D‐ADSC or U‐ADSC were seeded in collagen hydrogels deposited inside the microfluidic device and the rest of the assays were referred to 3D cultures. ARS, Alizarin red staining