FIGURE 10.

Orthogonal projection of Z‐stack confocal pictures. 3D cell culture was stained with DAPI and Phalloidin to observe cell nuclei and cytoskeleton, respectively. Specific protein secretion was targeted by immunostaining. BSP‐2 stained sample from 7D‐ADSC at 14 days of culture, OCN stained sample from 14D‐ADSC at 7 days of culture, DMP‐1 stained sample from 14D‐ADSC at 21 days of culture. Individual channels are shown together with the merged image, all of them at 40× magnification (scale bar 20 μm). ADSC, adipose‐derived stem cells; OCN, osteocalcein