TABLE 1.
Frequency of cells ex vivo and after culture with LACK and SLA
| Stimulus | Frequency (%)a of cell type after treatment
|
|||||
|---|---|---|---|---|---|---|
| CD4+ Tb | CD8+ T | CD3+ T | CD69+ T | CD45RO /CD4+ T | CD19+ B | |
| Ex vivo | 41.2 ± 1.6 | 20.0 ± 1.1 | 61.8 ± 2.5 | 2.3 ± 0.2 | 21.2 ± 0.8 | 9.6 ± 1.3 |
| LACKc | 52.4 ± 1.4d | 23.7 ± 1.3 | 78.8 ± 1.8d | 9.6 ± 0.4e | 20.4 ± 0.9 | 6.2 ± 1.0 |
| SLAc | 54.1 ± 2.6d | 21.7 ± 1.1 | 76.8 ± 6.0d | 18.4 ± 0.7f | 22.0 ± 1.8 | 10.2 ± 5.7 |
a
Mean ± standard error.
b
These values come from a total lymphocyte gate including small and large lymphocytes (R1 and R2) (Fig. 1).
c
Frequencies after 5 days of culture with the indicated antigen (LACK [20 μg/ml] and SLA [10 μg/ml]).
d
Significantly different from the frequency ex vivo using Student's t test and JMP with comparison of all pairs and a P value of <0.05.
e
Significantly different from the frequency ex vivo and with SLA using Student's t test and JMP with comparison of all pairs and a P value of <0.05.
f
Significantly different from the frequency ex vivo and with LACK using Student's t test and JMP with comparison of all pairs and a P value of <0.05.