FIGURE 4.

Gene expression clusters of temperature‐responsive genes with a significant overall temperature treatment effect, after 3 h (a) and after 24 h (b). Genes included in the figure on average responded significantly to a 5°C increase and/or decrease in temperature compared to the control constant 10°C in the four developmental weeks (N = 48 samples per panel). These genes showed a small but consistent response to temperature over time and/or had a large effect size in one or more weeks. For each heat map, columns correspond to the four development weeks sampled, and rows correspond to variance‐stabilizing transformation (vst) normalized and z‐transformed expression counts of each included gene, showing the difference in expression from 0 to 3 h (panel a, DEGs = 22 genes) or 0 to 24 h (panel b, DEGs = 274 genes) compared to the control, for the cold (blue column) and warm (red column) treatments. See Table S4.2 for included genes and corresponding statistics. For each identified gene expression profile cluster (indicated by grey scale bands on the left of each heatmap), the most significant GO terms are given (p < .01), with number of terms shown depending on the size of the cluster. For overview, the GO terms shown belong to different parent terms and very specific GO terms have been substituted for their parent term. See Table S5 for a complete list of overrepresented GO terms for each gene expression cluster