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. 2022 May 25;54(5):686–695. doi: 10.3724/abbs.2022047

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© The Author(s) 2021.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Effects of temperature, divalent metal ions, and NaCl concentration on activity of TpsAgo

(A) Effect of temperature on TpsAgo activity. (B) TpsAgo displays Co2+ and Mn2+ mediated ssDNA target cleavage. (C) Mg2+ concentration ranges required for TpsAgo activity. (D) Mn2+ and Co2+ concentration ranges required for TpsAgo activity. (E) Effect of NaCl concentration on TpsAgo activity. In all experiments, TpsAgo: guide: target ratio was 1:10:4. Error bars represent the standard deviation of three independent experiments.