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. 2022 Nov 16;54(11):1720–1730. doi: 10.3724/abbs.2022171

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© The Author(s) 2021.

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This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).

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USP14 interacts with NLRP3 and inhibits ubiquitination of NLRP3

(A) The interaction between USP14 and NLRP3 in AF cells was determined by coimmunoprecipitation. (B) The interaction between HA-USP14 and Flag-NLRP3 in transfected AF cells was determined by coimmunoprecipitation. (C) Effects of MG132 (10 μM) or vehicle on shUSP14-mediated NLRP3 expression. (D) AF cells were transduced with shUSP14 or shNC, and NLRP3 was immunoprecipitated and then immunoblotted with the indicated antibodies. * P<0.05, *** P<0.001 compared with shNC+vehicle.