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. 2022 Nov 16;54(11):1720–1730. doi: 10.3724/abbs.2022171

Figure 4 .

Figure 4

USP14 overexpression promotes AF cell pyroptosis by activating NLRP3

AF cells collected from IVDD patients were transduced with oeUSP14 or blank vector and treated with an NLRP3 inhibitor (INF39; 10 μM), and the expression of USP14 was examined by quantitative RT-PCR (A) and western blot analysis (B,C). (D,E) Cell pyroptosis was assessed by flow cytometry. (F) LDH release. (G,I) The expressions of NLRP3, active Caspase-1 (p20), MMP3, ADAMTS-5 and Aggrecan were measured by western blot analysis. (H) The concentrations of IL-1β and IL-18 were determined by ELISA. *** P<0.001 compared with vector; ### P<0.001 compared with oeUSP14.