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. 2022 Sep 20;54(9):1234–1243. doi: 10.3724/abbs.2022124

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© The Author(s) 2021.

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).

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Direct interaction of NAP1L1 and HDGF

(A) Immunofluorescence microscopy of NAP1L1, HDGF, and DAPI localization in colon cancer cells. Scale bar: 25 μm. (B,C) Co-IP assays were performed to examine the interaction between NAP1L1 and HDGF. Lysates were immunoprecipitated with anti-HDGF/anti-NAP1L1 antibodies or control IgG, and detected by western blot analysis using anti-NAP1L1 antibodies. (D) HDGF mRNA expression after si-NAP1L1-1/si-NAP1L1-2 transfection, normalized with ADP Ribosylation Factor 5. One-way ANOVA and Dunnett multiple comparison test. *** P<0.001. (E) HDGF protein expression after si-NAP1L1-1/si-NAP1L1-2 transfection. GAPDH was used as a loading control.