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. 2022 Sep 20;54(9):1234–1243. doi: 10.3724/abbs.2022124

Figure 5 .


Figure 5

NAP1L1 interacts with HDGF to recruit DDX5

(A) Immunofluorescence microscopy of HDGF, DDX5, and DAPI localization in colon cancer cells. Scale bar: 25 μm. (B,C) co-IP assay was performed to examine the interaction between DDX5 and HDGF. Lysates were immunoprecipitated with anti-HDGF/anti-DDX5 antibodies or control IgG, and detected by western blot analysis using anti-HDGF antibodies. (D) Expressions of NAP1L1, HDGF, and DDX5 were detected after transfection with sh-NAP1L1 with/without the HDGF overexpression plasmid. GAPDH was used as a loading control.