FIGURE 2.

Fluorescence and stable structural products from the oxidation of 6‐fluoroindole by toluene dioxygenase. (A) Relative fluorescence for each substituted indole substrate of toluene dioxygenase compared to indole. (B) Characterization of the extracted product of toluene‐grown Pseudomonas putida F1 incubated with 6‐fluoroindole by UV–vis spectroscopy. (C) ¹⁹F‐NMR spectrum (at 400 MHz ¹H) of extract dissolved in deuterochloroform (CDCl₃) (dt, J 5 Hz, 10 Hz, F‐11 reference). The inset shows the material analysed by NMR and UV–vis after extraction from culture media.