Figure3 .

MiR-146a-5p in telocytes decreases oxidative stress through the DUOX2 pathway

(A) The screening results of the enzymes involved in TC protection. (B) LPS increased MDA levels in both RAW264.7 and MLE-12 cells but reduced MDA levels in TCs. (C) DUOX2 was downregulated in TCs after LPS stimulation at both the mRNA and protein levels compared with those in RAW264.7 and MLE-12 cells. (D) Silencing of miR-146a-5p, but not miR-21a-3p, upregulated DUOX2 mRNA and protein expressions in TCs stimulated with LPS. Data are shown as the mean±standard deviation from six independent experiments (n=6). *P< 0.05.