Figure4 .

Mir-146a-5p regulates the expression of DUOX2 through the CREB1 pathway

(A) The expression of CREB1 in telocytes was decreased when treated with LPS and increased when TCs were treated with antagomiR-146a-5p. AntagomiR-146a-5p restored CREB expression in the presence of LPS. (B) A dual-luciferase reporter assay revealed that the miR-146a-5p mimic significantly reduced the CREB1 promoter activity. (C) AntagomiR-146a-5p significantly increased DUOX2 protein expression in TCs compared with cells transfected with the negative control. In the presence of antagomiR-146a-5p and LPS, KG-501 and 666-15 significantly reduced DUOX2 protein expression. Data are shown as the mean±standard deviation from six independent experiments (n=6). *P< 0.05.