Figure 1 .

GAS5 attenuated CS-induced airway remodeling

(A) The expression of GAS5 was measured by qRT-PCR in the lungs of mice with or without exposure to CS and injected with either a GAS5-overexpressing or an empty (control) lentiviral vector. Vector: empty vector. (B) The numbers of total cells and neutrophils in the BALF from the mice. (C) Histological analysis of the lung slices was performed by H&E staining. Average numbers of inflammation cells per field of view were also shown. Scale bar: 100 μm. (D) Morphology of the mouse lung slices was evaluated by Masson staining. Scale bar: 100 μm. (E) Lung slices were assessed by α-SMA staining. Scale bar: 100 μm. (F) Western blot analysis of the protein levels of α-SMA and collagen I (Col-I) in lungs and BALF, respectively. ** P<0.01.