Figure 5 .

MiR-217-5p overexpression and PTEN knockdown separately reversed the GAS5 -driven inhibition of airway inflammation and fibrosis

(A) GAS5-overexpressing MLE-12 cells were transfected with either the miR-217-5p mimic or PTEN siRNA (siPTEN) followed by treatment with 2.5% CSE. The expressions of IL6, TNFA, and TGFB mRNAs were measured by qRT-PCR. (B) GAS5-overexpressing fibroblasts were transfected with either the PTEN siRNA or miR-217-5p mimic, followed by CSE treatment. Both α-SMA and collagen I were quantified by western blot analysis. ** P<0.01.