Figure6 .

Upregulation of RETREG1-mediated ER-phagy alleviated ER stress in human NP cells

(A,B) Human NP cells were transfected with RETREG1 overexpressing lentivirus for 72 h, followed with 48 h GD treatment. The expressions of p-IRE1-α, IRE1-α, GRP78, caspase-12, and CHOP proteins were evaluated by western blot analysis, and relative quantification results are shown. (C,D) The NP cells transfected with RETREG1 siRNA were subjected to 48 h of GD treatment. The expressions of p-IRE1-α, IRE1-α, GRP78, caspase-12, and CHOP proteins were evaluated using western blot analysis, and relative quantification results are shown. (E,F) Results of immunofluorescence staining of p16 (green) and cleaved caspase-3 (red) proteins in human NP cells treated as mentioned above. The nuclei were stained with DAPI, scale bar: 50 μm. *P<0.05, **P<0.01.