Figure 5.

Relative contributions of distinct regulatory layers to the definition of the heat response. (a) Percentage of variance explained (R ²) by each MOFA2 factor (columns) across regulatory layers (rows) (left panel). Total contributions of each layer are summarized in the bar plot (right panel). (b) Scatter plot of latent factor 1 (x‐axis) and latent factor 2 (y‐axis) illustrating the variation described. Samples are colored according to treatment (C, T1, T3). (c) Lollipop plots showing top loading latent factor 1 (top) and latent factor 2 (bottom) features in descending order. Red and green indicate metabolome and proteome features, respectively. The sampling times correspond to the 40°C assay shown in Figure 1. (d,e) Enriched Mercator4 functional categories and top weight isoforms associated with latent factor 1 (e) and latent factor 2 (d). Functional categories marked with ‘*’ were renamed to subterms as follows because all top weight isoforms displayed belong to the same Mercator4 functional subterm: PS > photophosphorylation, solute transport > carrier‐mediated transport, nutrient uptake > nitrogen assimilation, DNA damage response > nucleotide excision repair. Positive, functional terms linked to samples with scores of >0; Negative, functional terms linked to samples with scores of <0.