FIGURE 4.

Confocal imaging analysis confirms HB‐7‐induced isatuximab binding to HB‐7‐bound RBCs. Binding of isatuximab and daratumumab (10 μg/ml) to RBCs in the absence or presence of HB‐7 (1 μg/ml) was evaluated by image‐based analysis. Green dots showing binding signals (AF488) on cell surface were visualized by microscope (60xW) (top panel of images) and number of positive spots were quantified (bottom graph). hIgG1, human immunoglobulin G1; RBC, red blood cell [Color figure can be viewed at wileyonlinelibrary.com]