Skip to main content
. 2001 May;69(5):3305–3314. doi: 10.1128/IAI.69.5.3305-3314.2001

FIG. 2.

FIG. 2

SDS-PAGE analysis of purified AS44–331 protein. Purification was achieved with a nickel column with E. coli BL21(DE3) containing pJKM82 as described in Materials and Methods. Lane 1, cell extract fall through in binding buffer; lane 2, wash buffer run through the nickel column; lane 3, elution of AS44–331 from the nickel column. The band represents approximately 10 μg of protein. Numbers on the left refer to molecular masses in kilodaltons.